Record Details

Secreted Proteases Control Autolysin-mediated Biofilm Growth of Staphylococcus aureus
IR @ Translational Health Science and Technology Institute
Title
Secreted Proteases Control Autolysin-mediated Biofilm Growth of Staphylococcus aureus
Creator
Chen, C. Krishnan, V. Macon, K.
Description
Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis.
Publisher
Date
2013
Type
Article PeerReviewed
Identifier
Chen, C. and Krishnan, V. and Macon, K. (2013) Secreted Proteases Control Autolysin-mediated Biofilm Growth of Staphylococcus aureus. Journal of Biological Chemistry, 288 (41). pp. 29440-29452.
Language