To study the influence of genes from Thinopyrum intermedium on traits affecting the bread-making quality of wheat, two derivatives from a putative disomic addition line in cultivar Vilmorin 27' were used in cytological, biochemical and molecular characterization. Cytological analysis suggested that one of the derivatives (Line-1) had a terminal deletion involving the long arm of chromosome 1D (2n=42, Del-1DL), and the other (Line-2) was a conventional addition line, but also carried the same deletion on chromosome 1D (2n=44, Thi+Del-1DL). Amplification and sequencing of high-molecular-weight glutenin subunit (HMW-GS) genes coded by the Th.intermedium chromosome in Line-2 indicated the presence of one x-type with an extra cysteine and four (rather than one) unique y-type genes. Rheological studies of Line-1 showed significantly lower dough strength compared to Vilmorin 27', confirming the recognized role of Glu-1D coded HMW-GSs. Line-2 showed significantly higher dough strength compared to the background cultivar, indicating a significant potential of Th.intermedium for improvement of bread-making quality in wheat.